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STAT 6 (aa650-670) (Signal Transducer and Activator of Transcription 6)

Cat no: S7972-40H

Supplier: United States Biological
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STATs (signal transducing activators of transcription) are mediators involved in cytokine signaling. Being the principle substrates of JAK kinases, phosphorylated STATs form head-to-tail homodimers. In response to a specific cytokine signal, STATs move to the nucleus and induce transcription. STAT6 is activated primarily by IL-4 and IL-13. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml for HRP/ECL detection ELISA: 0.05ug/ml Optimal dilutions to be determined by the researcher. Positive Control (supplied): Cell lysate of 2 x 10e6 untreated HepG2 cells. Supplied as a lyophilized powder. Reconstitute with 200ul sterile ddH2O. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S7972-40H
Reactivities: Human, Mouse, Canine
Hosts: Mouse
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from 2X PBS, PEG, sucrose, 0.09% sodium azide. Reconstitute with 1ml sterile, ddH2O.
P type: Mab
Isotype: IgG1
Purity: Purified by thiophilic adsorption and size exclusion chromatography.
Additional info: Recognizes human STAT6 at ~97kD. Species Crossreactivity: mouse, canine.

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