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Staphylococcus aureus alpha Hemolysin

Cat no: S7965-38B

Supplier: United States Biological
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Staphylococcus aureus alpha Hemolysin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. The toxin self-assembles to form first, a nonlytic oligomeric intermediate, and then, a mushroom-shaped homoheptamer structure. Heptamer oligomerization and pore formation is required for lytic activity. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, dH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S7965-38B
Hosts: Sheep
Applications: ELISA
Size: 1mg
Form: Supplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Reconstitute in 1ml 40-50% glycerol, dH2O.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes Staphylococcal alpha Hemolysin. Minimal reactivity with staphylococcal enterotoxins A through E and TSST.

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