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Src family, phosphorylated (Tyr416)

Cat no: S6504-25B

Supplier: United States Biological
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Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1: 1000 Optimal dilutions to be determined by the researcher. Positive Control: RIPA cell lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S6504-25B
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in PBS, 0.05% sodium azide.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Src protein-tyrosine kinase structure and regulation, Roskoski, Robert (2004) Biochem. Biophys. Res. Commun., 324: 1155-64 (2004), 2. Molecular interdiction of Src-family kinase signaling in hematopoietic cells, Geahlen, Robert L, et al. (2004) Oncogene, 23: 8024-32 (2004), 3. Role of STATs as downstream signal transducers in Src family kinase-mediated tumorigenesis, Silva, Corinne M (2004) Oncogene, 23:8017-23 (2004); 4. Structure and regulation of Src family kinases, Boggon, Titus J and Eck, Michael J (2004) Oncogene, 23: 7918-27 (2004), 5. The interplay between Src family kinases and receptor tyrosine kinases, Bromann, Paul A, et al (2004) Oncogene, 23:7957-68 (2004)
Additional info: Recognizes vertebrates phospho-Rsk3 (Thr353/Thr356). Sequence homology: mouse.

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