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Sprouty 4 (Sprouty-4, Sprouty Homolog 4, Spry 4, SPRY4, SPRY-4)

Cat no: S5620-25A

Supplier: United States Biological
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Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Cellular Localization: Cytoplasmic Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S5620-25A
Reactivities: Human
Hosts: Rabbit
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 100ug
Form: 0.15M Sodium Chloride, 0.02M Potassium Phosphate [pH 7.2]
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: Sasaki A et al. Identification of a dominant negative mutant of Sprouty that potentiates fibroblast growth factor- but not epidermal growth factor-induced ERK activation. J Biol Chem 276:36804-8 (2001).
Additional info: Species Crossreactivity: Expected to crossreact with Human (100% identity with immunogen) and Mouse (88% identity with immunogen) due to sequence homology.

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