SPIR1 belongs to the family of Wiskott-Aldrich homology region 2 (WH2) proteins involved in actin organization. The C-terminal region of SPIR1 encodes a zinc finger structure highly homologous to FYVE motifs. The protein also possesses a region highly homologous with other Spir family proteins located adjacent (N-terminal) to the modified FYVE domain and is designated as Spir-box. The protein colocalized specifically with Rab11 GTPase, which is localized at the trans-Golgi network (TGN), post-Golgi vesicles, and the recycling endosome. SPIR1 actin organizer is targeted to intracellular membrane structures by its modified FYVE zinc finger and is involved in vesicle transport processes, providing a novel link between actin organization and intracellular transport. The human SPIR1 gene is localized in the chromosomal region 18p11.21.
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.