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SPIB (Spi-B Transcription Factor (Spi-1/PU.1 Related), Spi-B, Transcription Factor Spi-B)

Cat no: S5490

Supplier: United States Biological
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Spi-B is a transcription factor belonging to a subfamily of ETS transcription factors with a conserved ETS domain that mediates specific DNA binding. Spi-B may form homotypic interactions and is known to interact with IRF4, CREBBP, EP300, SPI1:PU.1 related, JUN and TBP. As a sequence specific transcriptional activator it binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Two isoforms of Spi-B are reported. Spi-B is usually expressed in plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells and promotes their development and block viral replication. Spi-B is not expressed in T-cells or granulocytes but may be present in stem cell populations of the liver and B-cells where it helps in B-cell receptor (BCR) signaling, normal B-cell development and antigenic stimulation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human spleen lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S5490
Reactivities: Human
Hosts: Rabbit
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: 1. Ray, D. et al. Molec. Cell. Biol. 12:4297-4304 (1992). 2. Ray-Gallet, D. et al. Biochem. Biophys. Res. Commun. 223:257-263 (1996). 3. Yamamoto, H. et al. Cell Growth Differ. 13:69-75 (2002). 4. Schotte R. et al. Blood. 101:1015-1023 (2003).
Additional info: Recognizes human Spi-B. Species Crossreactivity: chimpanzee, rhesus monkey. Species sequence homology: feline, bovine, canine, equine, mouse, rat.

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