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Spi-1, CT (Spi1, Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1, Transcription Factor SPI-1, Pu1)

Cat no: S5490-10

Supplier: United States Biological
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Spleen focus forming virus [SFFV] proviral integration oncogene (spi-1) is a mammalian transcription factor critically involved in a widespread role throughout hematopoiesis in many species. The zebrafish orthologue of spi-1 marks a population of rostral lateral plate mesoderm cells during development fated for myeloid differentiation. Spi-1 gene expression has been demonstrated to be largely recapitulated in transgenic zebrafish embyos via injection of an upstream promoter. Zebrafish are a powerful tool for the study of vertebrate hematopoiesis, as a result of possessing a robust primitive myeloid pathway that generates both granulocytes and macrophages. Previously known as pu1, spi-1 has been demonstrated strong functional conservation between zebrafish primitive myelopoiesis and mammalian definitive myelopoiesis. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:5000-1:20,000 Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Control Peptide: S5490-10P Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S5490-10
Reactivities: Zebrafish/Fish
Hosts: Rabbit
Applications: ELISA, Western Blot
Size: 250ul
Form: Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Bukrinsky, A et al (2009) Blood. 113(9):2038-2046. 2. Lieschke, GJ et al (2002) Dev. Biol. 246(2):274-295. 3. Ward, AC et al (2003) Blood. 102(9):3238-3240. 4. Su, F et al (2007) Zebrafish 4(3):187-199.
Additional info: Recognizes zebrafish Spi-1.

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