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SPAG16 (Sperm Associated Antigen 16 aa139-152)

Cat no: S5401-36

Supplier: United States Biological
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Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively (Zhang et al., 2007 [PubMed 17699735]). Applications: Suitable for use in ELISA. Other applications have not been tested. Recommended Dilutions: Peptide ELISA Titer: 1:16,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S5401-36
Reactivities: Human
Hosts: Goat
Applications: ELISA
Size: 100ug
Form: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by peptide affinity chromatography.
Alternative names: SPAG16, WDR29, WD repeat domain 29, sperm-associated WD repeat protein, sperm associated antigen 16, PF20, MGC87036, FLJ37717, FLJ22724, DKFZp666P1710
References: 1. Zhang Z, Zariwala MA, Mahadevan MM, Caballero-Campo P, Shen X, Escudier E, Duriez B, Bridoux AM, Leigh M, Gerton GL, Kennedy M, Amselem S, Knowles MR, Strauss JF,A heterozygous mutation disrupting the SPAG16 gene results in biochemical instability of central apparatus components of the human sperm axoneme.Biology of reproduction 2007 Nov 77 (5): 864-71.
Additional info: Recognizes SPAG16. Species sequence homology: human, canine, bovine. This antibody is expected to recognize isoform 1 and 2 (NP_078808.3; NP_001020607.1).

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