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SOX9, Phosphorylated (Ser181) (Transcription Factor SOX-9)

Cat no: S5364-26E

Supplier: United States Biological
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SOX-9 is a member of the family of SOX (Sry-type high mobility group box) gene that were first identified on the basis of region with high homology to that of Sry (Sex-determining region Y). It is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. Phosphorylation of SOX-9 by PKA increases its DNA binding and transcriptional activity. SOX-9 regulates expression of chondrocyte phenotype related genes. IL-1 and TNF-a can cause a marked and rapid decrease in the levels of SOX9 mRNA and/or protein in chondrocytes, thus strongly inhibit the expression of genes for cartilage extracellular matrix proteins. The down-regulation of SOX-9 may have a crucial role in inhibiting expression of the cartilage phenotype in inflammatory joint diseases. Applications: Suitable for use in Western Blot and Dot Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml Dot Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S5364-26E
Reactivities: Human
Hosts: Rabbit
Applications: Western Blot
Size: 25ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Koopman, S. et al. J Endocrinol 169, 573 (2001). 2. Crombrugghe, B. et al. Matrix Biol 19, 389 (2000). 3. Murakami, S. et al. J Biol Chem 275, 3687 (2000). 4. Sekiya, I. et al. J Biol Chem 275, 10738 (2000). 5. McDowall, S. et al. J Biol Chem 274, 24023 (1999). 6. Wright, E. et al. Nucleic Acids Res 21, 744 (1993).
Additional info: Recognizes human SOX-9, phosphorylated at Serine 181.

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