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Sorbitol Dehydrogenase (SDH, SORD, SORD1, L Iditol 2 Dehydrogenase, L-iditol 2-dehydrogenase)

Cat no: S5350-30

Supplier: United States Biological
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Sorbitol dehydrogenase (SDH), a member of the medium-chain dehydrogenase/reductase protein family and the second enzyme of the polyol pathway of glucose metabolism, converts sorbitol to fructose strictly using NAD(+) as coenzyme. Sorbitol dehydrogenase catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications. The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor. It has been noted that the polyol pathway is particularly active in hyperglycemic states. Although SORD is closely related to the class I long-chain alcohol dehydrogenases, it differs in substrate specificity, catalyzing polyols such as sorbitol and xylitol but having no activity towards primary alcohols. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S5350-30
Reactivities: Human, Mouse
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris-saline, pH 7.2. 0.5% BSA, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: Karacaoglan V & Ozer I Steady-state kinetic properties of sorbitol dehydrogenase from chicken liver. Comp Biochem Physiol B Biochem Mol Biol 140:309-12 (2005). PubMed: 15649778
Additional info: Species Crossreactivity: Reacts with Mouse. Predicted to react with Human, Rat, Chicken and canine due to sequence homology.

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