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Solute Carrier Family 22 (Organic Cation Transporter) Member 1, SLC22A1)

Cat no: S5329-36D

Supplier: United States Biological
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SLC22A1 (solute carrier family 22 member 1), also called OCT1, is a 554 amino acid (aa), 70-80kD 12-transmembrane glycoprotein of the SLC22 family of polyspecific organic cation transporters (OCT). SLC22A1 is expressed in epithelial cells and some neurons, with detection on hepatocyte sinusoidal membranes and enterocyte basolateral membranes. It transports selected cations bidirectionally and is inhibited by non-transported cations. Human SLC22A1 shares 78% aa identity with mouse and rat SLC22A1. In humans, three inactive isoforms of 506, 483 and 353 aa, lack C-terminal exons 5, 5 and 6, or 3 and 6, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml using Jurkat human acute T cell leukemia cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S5329-36D
Reactivities: Human
Hosts: Mouse
Applications: Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from PBS, 5% trehalose Reconstitute with 200ul sterile PBS.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein A affinity chromatography.
Additional info: Recognizes human SLC22A1.

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