Superoxide dismutatses catalyzes the conversion of single electron reduced species of molecular oxygen to hydrogen peroxide and oxygen. Cu, Zn superoxide dismutase (SOD1) is widely distributed and comprises 90% of total SOD. Mutations in SOD1 cause a form of familial ALS. Oxidative modifications and aggregation of SOD1 has been associated with Alzheimer's and Parkinson's disease.
Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 0.5-4ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Immunohistochemistry: Frozen
Immunoprecipitation: 20ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.