Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems. The SNAP-Tag is a self-labeling protein tag that forms a highly stable, covalent thioether bond with fluorophores and other substituted groups on its benzyl guanine substrate. The expressed SNAP-tag fusion proteins can be used in many different assay formats depending upon the labeling substrate employed.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 0.05-0.2ug/ml
Western Blot: 0.1-1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.