SUMOylation is a reversible post-translational modification of numerous cellular proteins mediated by SUMO proteins. SUMOylation regulates various cellular processes which include nuclear transport, chromatin structure, DNA repair, protein stability and apoptosis. SUMO consists of unique, highly variable N-terminal extensions, a conserved Ubiquitin domain and C-terminal diglycine cleavage:attachment site. SUMO-3, a 20kD protein, is a novel SUMO paralogue which is highly homologous to Ubiquitin and is a human homolog of S.cerevisiae SMT3. It has the ability to form poly (SUMO) chains along with SUMO-2. Localized throughout the nucleoplasm, it modifies various proteins including Topoisomerase II, C:EBPbeta, and PML. SUMO-3 along with SUMO-1 mediates PIASy-enhanced modification of C:EBPalpha and inhibits transcriptional synergy. SUMO3 might be involved in structure and function of eukaryotic kinetochore.
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.