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Smoothelin (SMSMO, SMTN, FLJ35365, FLJ38597)

Cat no: S1014-62C

Supplier: United States Biological
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Smoothelin is one of the smooth muscle cell specific proteins such as calponin, alpha smooth muscle cell actin, SM22alpha and smooth muscle myosin heavy chain isoforms. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. It colocalizes with actin stress fibers, this distribution indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Two major isoforms of smoothelin have been reported, a 59kD smoothelin-A in visceral smooth muscle cells and a 110kD smoothelin-B in vascular smooth muscle cells. Both gene products are encoded by a single copy gene which is located on chromosome 22 and regulated by a dual promoter system in a tissue specific manner. The variation in the smoothelin proteins is achieved by alternative splicing in the calponin homology domain. Antibodies to Smoothelin are powerful tool in the evaluation of smooth muscle cell differentiatiation as occurs in atherosclerotic lesions. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50 Flow Cytometry: 1:50 Immunohistochemistry (frozen): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S1014-62C
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.02% sodium azide, 0.1% BSA.
P type: Mab
Isotype: IgG1
Purity: Purified
References: 1. Loop, van der F., et al., J. Cell. Biol. 34: 401 (1996). 2. Eys, van G., et al., Cell Struct. Funct. 22: 65 (1997). 3. Loop, van der F., et al., Arterioscler. Thromb. Vasc. Biol. 17: 665 (1997). 4. Johnson, J., et al., Arterioscler. Thromb. Vasc. Biol. 21: 1146 (2001). 5. Rensen, S., et al., Cardiovasc. Res. 55: 850 (2002). 6. Maeng, M., et al., Scand. Cardiovasc. J. 37: 34 (2003). 7. Saleem, M., et al., Am. J. Physiol. Renal Physiol. 295: F959 (2008).
Additional info: Recognizes human Smoothelin. Recognizes both Smoothelin-A and Smoothelin-B. The epitope is located within a stretch of 30aa in exon 11 (aa56-86 of Smoothelin-A and aa512-542 of Smoothelin-B).

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