The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.
Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Proximity Ligation Assay. Other applications not tested.
Recommended Dilution:
Immunoprecipitation: 2-5ug/mg lysate
Western Blot: 1:2000-1:10,000
Proximity Ligation Assay: 1:200-1:2000
Immunohistochemistry: 1:250-1:2000
Immunohistochemistry (paraffin): 1:10-1:2000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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