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Smad4, CT (DPC4, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Cat no: S1014-55D

Supplier: United States Biological
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SMAD4 is a member of the Mothers Against Dpp (MAD)-related family of proteins. SMAD4 is functionally distinct among the SMAD family, and is required for the assembly and transcriptional activation of diverse, SMAD-DNA complexes. SMAD4 appears to be not regulated by phosphorylation, but acts as a common mediator of TGF-beta, activin, and bone morphogenetic protein signaling responses. SMAD4 is frequently inactivated in pancreatic, biliary and colorectal tumors. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:20 Western Blot: 1:1000-1:5000 Immunoprecipitation: 1:30 Immunohistochemistry (Paraffin): 1:100-250 Immunocytochemistry: 1:100-250 Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Recommended Positive Control: SHSY5Y cell lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S1014-55D
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Eppert, K., et al., Cell 86: 543-552 (1996). 2. Derynck, R., et al., Curr. Biol. 6: 1226-1229 (1996). 3. Hudson, J., et al., Development 125: 1407-1420 (1998). 4. Lagna, G., et al., Nature 383: 832-836 (1996). 5. Hahn, S., et al., Science 271: 350-353 (1996). 6. Goggins, M., et al., Cancer Res. 58: 5329-5332 (1998).
Additional info: Recognizes human SMAD4 at ~65kD. Species Crossreactivity: mouse and rat

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