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Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Cat no: S1014-53Z

Supplier: United States Biological
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SMAD3, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is estimated to account for at least 80% of all TGF-beta-mediated response. Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their c-terminal two extreme serines in the SSXS motif. The phosphorylated R-SMAD translocate into nucleus, where they regulate transcription of target genes. SMAD3 signal transduction appears to be important in the rgulation of muscle-specific genes. Loss of SMAD3 is a feature of pediatric T-cell lymphoblastic leukemia, while upregulation of SMAD3 may be responsible for TGFB hyperresponsiveness observed in scleroderma. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunohistochemistry-M, Immunocytochemistry, Immunoprecipitation.. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:10000 Immunohistochemistry: 1:100-1:250 Immunohistochemistry-M: 1:100-1:250 Immunocytochemistry: 1:100-1:250 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: S1014-53Z
Reactivities: Human, Rat
Hosts: Rabbit
Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as liquid in PBS, 0.09% sodium azide.
P type: Mab
Purity: Supernatant
References: 1. Yang X, et al. Devel Biol 95:3667-3672, 1998, 2. Preobrazhenska O, et al. Oncogene 21:5660-5664, 2002, 3. Qing C et al. J Biol Chem 275:38802-38812, 2000, 4. Han SU, et al. Oncogene 23:1333-1341, 2004.
Additional info: Recognizes human SMAD3. Species Crossreactivity: rat.

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