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Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Cat no: S1014-53M

Supplier: United States Biological
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Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-beta signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include Smad1, 2, 3, 5 and 8, the common-mediator SMAD (co-SMAD), SMAD4, and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7. Once in the nucleus, SMADs can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-beta, SMAD2 and SMAD3 become phosphorylated at their carboxyl termini (Ser465 and 467 on SMAD2; Ser423 and 425 on SMAD3) by TGF-beta Receptor I. Phosphorylated SMAD 2/3 can complex with SMAD4, translocate to the nucleus and regulate gene expression. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:5,000 Immunohistochemistry (paraffin): 1:100-250 Immunocytochemistry: 1:100-250 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cell lysate Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S1014-53M
Reactivities: Human, Rat
Hosts: Rabbit
Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
Additional info: Recognizes human SMAD3. Species Crossreactivity: rat.

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