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SLP 76, phosphorylated (Tyr113) (SH2-domain Leukocyte Protein)

Cat no: S1014-20M

Supplier: United States Biological
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The activation of protein tyrosine kinases is a critical event in T cell antigen receptor (TCR)-mediated signaling. One substrate of the TCR-activated protein tyrosine kinase pathway is a 76-kD protein (SLP-76) that associates with the adaptor protein Grb2. Northern analysis demonstrates that pp76 mRNA is expressed solely in peripheral blood leukocytes, thymus, and spleen; and in human T cell, B cell and monocytic cell lines. In vitro translation of pp76 cDNA gives rise to a single product of 76kD that associates with a GST/Grb2 fusion protein, demonstrating a direct association between these two molecules. Results demonstrate that this novel protein is likely to play an important role in TCR-mediated intracellular signal transduction (1). It has been shown that both SLP-76 and ZAP70 co-immunoprecipitate with Shb in Jurkat T cells and that Shb and Vav co-immunoprecipitate when cotransfected in COS cells. Results indicate that upon TCR stimulation, Shb is targeted to these lipid rafts where Shb aids in recruiting the SLP-76-Gads-Vav complex to the T cell receptor zeta-chain and ZAP70 (2). Tyrosine phosphorylation of SLP-76 at position 113 or 128 was sufficient for it to associate with the N-terminal SH2 of p85. This data suggest that SLP-76 may play a role in PI3K signaling pathways (3). Applications: Suitable for use in Western Blot, Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:5000-10,000 Flow cytomtery: 1:10 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: S1014-20M
Reactivities: Human
Hosts: Rabbit
Applications: Flow Cytometry, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
Alternative names: SH2 domain-containing leukocyte protein of 76kD, SLP-76 tyrosine phosphoprotein, SLP76
References: 1. Jackman JK, et al J Biol Chem 270(13):7029-32, 1995 2. Lindholm CK et al. Eur J Biochem 269(13):3279-88, 2002 3. Shim EK, et al. FEBS Lett 575(1-3):35-40, 2004
Additional info: Recognizes human SLP-76 phosphorylated on Tyr113.

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