SLAIN1 (SLAIN motif-containing protein 1) is a 61kD (predicted) member of the SLAIN motif containing family of proteins. It is expressed in embryonic stem cells plus cells of the neural tube, and also appears in multiple adult tissues. Its function is unclear. Mouse SLAIN1 is 579aa in length. It contains a coiled-coil region (aa21-56), a Pro-rich segment (aa64-160), and one C-terminal SLAIN (SerLeuAlaIleAsn) motif. There are two potential splice variants. One shows an alternate start site at Met262, while another shows a 50aa insertion after Gln231. Over aa262- 483, mouse SLAIN1 shares 96% and 92% aa identity with rat and human SLAIN1, respectively.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.