Home  >  Products  >  SIRT 5 (Sirtuin 5, Sirtuin-5, NAD-dependent Deacetylase Sirtuin-5, SIR2-like Protein 5 SIR2L5) (Biotin)

SIRT 5 (Sirtuin 5, Sirtuin-5, NAD-dependent Deacetylase Sirtuin-5, SIR2-like Protein 5 SIR2L5) (Biotin)

Cat no: S1013-88J6

Supplier: United States Biological
0 reviews | Write a Review Pencil
Sirtuin 5 (SIRT5; SIR2-like protein 5) is a 28-35kD class III member of the sirtuin protein family. It is found in the mitochondrial matrix and is involved in ammonia detoxification. In particular, it deacetylates carbamoyl phosphate synthetase 1/CPS1, thus activating it and allowing for ammonia condensation with bicarbonate and ATP. This is a crucial step in urea formation during prolonged fasting or the use of a high-protein diet. Human SIRT5 is 310aa in length. It possesses a sertuin-type deacetylase domain (aa41-309) that contains an NAD binding motif (Thr276:Glu277:Pro292). There are three potential isoform variants that show aa substitutions at the C-terminus. One contains a 14aa substitution for aa286-310, a second shows a 15aa substitution for aa287-310, and the third shows a 17aa substitution for aa207-310. Full-length human and mouse SIRT5 share 86% aa identity. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S1013-88J6
Reactivities: Human
Hosts: Sheep
Applications: Western Blot
Conjugates: Biotin
Size: 50ug
Form: Supplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes human SIRT5. Species sequence homology: mouse (86%).

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*

Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave