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Sialoprotein, Bone (BSP, Bone Sialoprotein 2, BSP2, Cell-binding Sialoprotein, Integrin-binding Sialoprotein)

Cat no: S1013-34P

Supplier: United States Biological
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Bone sialoprotein (BSP) is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70-80kD. The protein has been found in osteoblasts and osteocytes. BSP is synthesized and secreted by bone-, dentin- and cementum-forming cells. Diseases concerning the bone turnover, pathological bone alterations as well as the rapid increase of osteoporosis make it necessary to establish new markers. Bone sialoprotein is discussed as a potential serum marker for monitoring bone remodelling. Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: ELISA: 1ug/ml RIA: 20ng/ml Flow Cytometry: 2ng/ml Immunohistochemistry: 1:100. Paraffin Western Blotting: 1:1000-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S1013-34P
Reactivities: Human
Hosts: Chicken/Bird
Applications: ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Size: 20ul
Form: Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute in ddH2O.
P type: Pab
Isotype: IgY
Purity: Serum
References: 1. Karmatschek M, Maier I, Seibel MJ, Woitge HW, Ziegler R, Armbruster FP. Improved purification of human bone sialoprotein and development of a homologous radioimmunoassay. Clin Chem 1997; 43:2076-82. 2. Bellahcene A, Van Riet, I, de Greef C, Antoine N, Young MF, Van Camp B, Castronovo V. Bone sialoprotein mRNA and protein expression in human multiple myeloma cell lines and patients. Br J Haematol 2000; 111:1118-21. 3. Adwan H, Bauerle T, Najajreh Y, Elazer V, Golomb G, Berger MR.:Decreased levels of osteopontin and bone sialoprotein II are correlated with reduced proliferation, colony formation, and migration of GFPMDA- MB-231 cells.Int J Oncol. 2004 May;24(5):1235-44.
Additional info: Recognizes human Bone Sialoprotein (native protein). There were no cross reactivities obtained with human Osteonectin, human Osteopontin and human Alkaline Phosphatase.

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