PTPN6 is a 68kD cytosolic SH2 domain- containing protein tyrosine phosphatase and member of the protein tyrosine phosphatase sub-family, which acts as a critical regulator of haematopoietic cell behavior and signalling cascadesSHP1 binds via two tandem N-terminal Src homolog (SH2) domains to phosphotyrosine residues on a number of substrates, but can also associate with the insulin receptor and JAK2 in an SH2-independent manner. SHP1 has been shown to act as a negative regulator of cytokine signal transduction through the dephosphorylation of cytokine receptors, including those for interleukin-3 and -4, the stem cell factor c-kit and colony stimulating factor-1 (CSF-1) in haematopoietic cells, but also acts as a positive regulator of IFNgamma and epidermal growth factor (EGF) signalling in epithelial cells.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:128,000
Western Blot: 0.1-0.3ug/ml, detects bands of approximately 60-70kD in human liver and bone marrow cell lysates.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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