SH3PXD2B is a novel adaptor protein; also known as SH3 and PX domain-containing protein 2B, (Courtneidge, 2005). It is closely related to Tks5 (gene symbol SH3PXD2A) and like Tks5, appears to play a role in the formation and function of podosome/invadopodia based on studies of Src-transformed fibroblasts (Buschman, 2009). Additionally, SH3PXD2B may be involved in the recruitment of MT1-MMP and ECM degradation.
SH3PXD2B is broadly expressed and localizes in the cytoplasm of the podosome.
Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 3ug/ml. 50ug of NIH3T3 IP cell lysate was resolved via SDS-PAGE, transferred to PVDF and probed with 3ug/ml S1013-33A. Proteins were visualized using an IgG (HRP) anti-rabbit that does not recognize denatured IgG. Detection using a chemiluminescence system.
Immunoprecipitation: 10ug immunoprecipitates 500ug of NIH3T3 whole cell lysate
Immunocytochemistry: 1:3000
Optimal dilutions to be determined by the researcher.
Positive Control:
NIH3T3 cell lysate
Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.