sFRP2, otherwise known as SARP-1 (secreted apoptosis-related protein 1), is a member of the secreted frizzled-related protein family which interact directly with Wnt, to act as soluble modulators of Wnt signalling. Several studies have linked the epigenetic silencing of the sFRP2 gene with cancer, including breast cancer, colorectal cancer, gastric cancer, renal cell carcinoma and acute myeloid leukemia.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:32,000
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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