LKB1 (also STK11 and JBS) is a 55kD member of the CAMK Ser/Thr protein kinase
family. It is ubiquitously expressed, and considered to be a tumor suppressor. LKB1 is
both nuclear and cytoplasmic, and appears to contribute to cell polarization, G1 cell cycle
arrest and Wnt signaling. It is known to complex with STRAD and CAB39/MO25, and
phosphorylate PTEN plus p53. Human LKB1 is 433 amino acids (aa) in length. It contains
a protein kinase domain (aa 49-309) and a prenylation motif (CysLysGlnGln) over
aa 430-433. There are two potential isoforms of LKB1. One shows a nine aa insertion after
Tyr126, while another shows a 34 aa substitution for aa 371-433. Over aa 242-433,
human and mouse LKB1 are identical in aa sequence.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:164000
Western Blot: 0.3-1ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control:
Human Ovary lysates
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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