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SC5b-9 (Complement SC5b-9 Complex)

Cat no: S7850-26

Supplier: United States Biological
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Both the classical and alternative complement pathways result in the formation of the cytolysis inducing C5b-9 complex. This complex is composed of 190kD C5b which is bound to cells via 71kD C9. Sublytic assembly of C5b-9 on plasma membranes induces cell cycle activation and survival. The binding of C5b-9 to the 75kD S-protein (or vitronectin) in the fluid phase prevents C5b-9 from assembling on the plasma membrane, deactivating it and forming the SC5b-9 complex. SC5b-9 is stable in vitro and is therefore a reliable indicator of terminal complement pathway activation. Applications: Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control: Kidney from post streptoccal glomerulonephritis patients. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, (HRP) Pab Gt xMo I1904-19C: IgG, (HRP) Pab Gt xMo Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S7850-26
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in borate buffered saline, 0.1% sodium azide.
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein A affinity chromatography.
References: 1. Biesecker, G. (1990) The complement SC5b-9 complex mediates cell adhesion through a vitronectin receptor. J. Immunol. 145:209. 2. Greenstein, J. et al. (1995) The kinetics and distribution of C9 and SC5b-9 in vivo: effects of complement activation. Clin. Exp. Immunol.100:40-6. 3. Rus H, et al. (2005) C5b-9 complement complex in autoimmune demyelination and multiple sclerosis: dual role in neuroinflammation and neuroprotection. Ann Med. 37:97-104
Additional info: Recognizes the human SC5b-9 complex of around 330kD.

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