Home  >  Products  >  SAE2 (SUMO-activating Enzyme Subunit 2, Anthracycline-associated Resistance ARX, Ubiquitin-like 1-activating Enzyme E1B, UBA2, UBLE1B, HRIHFB2115)

SAE2 (SUMO-activating Enzyme Subunit 2, Anthracycline-associated Resistance ARX, Ubiquitin-like 1-activating Enzyme E1B, UBA2, UBLE1B, HRIHFB2115)

Cat no: S8025-90H

Supplier: United States Biological
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Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes. The conjugation of SUMO-1, SUMO-2 and SUMO-3 onto target proteins requires the concerted action of the specific E1-activating enzyme SAE1:SAE2, the E2-conjugating enzyme Ubc9, and an E3-like SUMO ligase. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: HeLa cell lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S8025-90H
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by Protein G affinity Chromatography.
References: 1. Tatham MH, Kim S, Jaffray E, Song J, Chen Y, Hay RT. Unique binding interactions among Ubc9, SUMO and RanBP2 reveal a mechanism for SUMO paralog selection. Nat Struct Mol Biol. 2004 Dec 19; [Epub ahead of print] 2. Identification of the Enzyme Required for Activation of the Small Ubiquitin-like Protein SUMO-1.Joana M. P. Desterro, Manuel S. Rodriguez, Graham D. Kemp, and Ronald T. Hay. J Biol Chem, Vol. 274 (15): 10618-10624 (1999).
Additional info: Recognizes human SAE2. Species Crossreactivity: mouse and rat.

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