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Cat no: S6609-96D

Supplier: United States Biological
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The recombinant protein over-expression systems with well-known epitope tags are powerful strategies in checking and purifying the interest proteins, which are expressed in certain vectors containing the particular tagged fragments. The small tags are used to be cloned 50 along with the target proteins, and the antibodies against those fusion-tags are used to monitor fusion protein expression and purification. S-Tag is an epitope tag composed of a 15aa peptide (KETAAAKFERQHMDS) derived from the pancreatic ribonuclease A. This antibody can be used to check S- tagged expressed proteins. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S6609-96D
Hosts: Rabbit
Applications: Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Binz HK et al (2004) J. Nat. Biotechnol. 22(5), 575-582 2. Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. 3. Lu Q et al (1997) J. Gene 200(1-2), 135-144. 4. Malby RL et al (1993) J. Proteins 16(1), 57-63. 5. Wilson, I. A., Niman, H. L., Houghten, R. A., Cherenson, A. R., Connolly, M. L., and Lerner, R. A. (1984) Cell 37, 767-778.
Additional info: Recognizes S-Tag in all species.

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