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S-phase Kinase-associated protein 1A (Skp1)

Cat no: S1014-16B

Supplier: United States Biological
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S-phase kinase-associated protein 1A (Skp1) is a component of the SCF ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Skp1 interacts directly with cullin 1 and F-box proteins in the SCF complex. It is also involved in the control of beta-catenin levels and the activity of beta-catenin dependent TCF transcription factors. Applications: Suitable for use in ELISA, Immunoprecipitation, and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:2,000-1:10,000 Western Blot: 1:500-1:1,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S1014-16B
Reactivities: Human
Hosts: Rabbit
Applications: ELISA, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid, 0.01% sodium azide.
P type: Pab
Purity: Serum
References: 1. Michel, J.J., et al., (1998), "Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A", Cell Growth Differ., 9 (6), 435-449. 2. Ohta, T., et al., (1999), "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity", Mol. Cell., 3 (4), 535-541. 3. Zhang, H., et al., (1995), "p19Skp1 and p45Skp2 are essential elements of the cyclin A-CDK2 S phase kinase", Cell, 82 (6), 915-925. 4. Bai, C., et al., (1996), "SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box", Cell, 26:86(2), 263-274. 5. Connelly, C., et al., (1996), "Budding yeast SKP1 encodes an evolutionarily conserved kinetochore protein required for cell cycle progression", Cell, 86(2), 275-285. 6. Feldman, R.M., et al., (1997), "A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p", Cell, 91(2), 221-230. 7. Liu, J., et al., (2002), "NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases", Mol. Cell, (6), 1511-1518.
Additional info: Recognizes human SKP1.

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