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S-phase Kinase-associated protein 1A (Skp1)

Cat no: S1014-16A

Supplier: United States Biological
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S-phase kinase-associated protein 1A (Skp1) is a component of the SCF ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Skp1 interacts directly with cullin 1 and F-box proteins in the SCF complex. It is also involved in the control of beta-catenin levels and the activity of beta-catenin dependent TCF transcription factors Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:250 Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S1014-16A
Reactivities: Human
Hosts: Rabbit
Applications: ELISA, Western Blot
Size: 1mg
Form: Supplied as a liquid in PBS, pH 7.2.
P type: Pab
Purity: Purified by Protein G affinity chromatography.
References: Schulman, B.A. et al. (2000) Nature 408,381-386. Zheng, N. et al. (2002) Nature 416,703-709. Cardoso, T. & Pagano, M. (2006) Nat. Rev. Mol. Cell Biol. 5,739-751.
Additional info: Recognizes human Skp1

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