PRDM2 (PR Domain Containing Protein 2, G3B, KMT8, MTE-binding Protein, MTB-ZF, PR Domain Zinc Finger Protein 2, PR-domain Zinc Finger Protein 2, Retinoblastoma Protein-binding Zinc Finger Protein, Retinoblastoma Protein-interacting Zinc-finger Protein, RI

PRDM2, a member of nuclear:histone methyltransferase superfamily, consists of an N-terminal PR domain, an Rb-binding motif, 8 Kruppel-like zinc finger motifs, Nuclear hormone-receptor binding motif and a C-terminal PR-domain binding motif. Ubiquitously expressed in low levels, PRDM2 exists in 2 isoforms, a 280kD RIZ1 containing PR-domain and a 250kD truncated RIZ2 that lacks PR-domain. A tumor suppressor with a prominent role in cell growth and differentiation, PRDM2 binds to Retinoblastoma protein, estrogen receptor, GATA-3 and TPA-responsive element (MTE) of Heme-oxygenase-1 gene and influences pathogenesis of Retinoblastoma, transcriptional regulation during neuronal differentiation, transactivation of Heme-oxygenase-1 gene and specific effector of estrogen action. It is inactivated or altered due to mutations and hypermethylation in many types of human cancers suggesting a potential biomarker.\n\nApplications:\nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:100-1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P5750-10C

Size

100ul

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid, 0.025% sodium azide, 50% glycerol.

P Type

Pab

Purity

Serum.

Isotype

IgG

References

1. Buyse,I.M., Shao,G. and Huang,S. The retinoblastoma protein binds to RIZ, a zinc-finger protein that shares an epitope with the adenovirus E1A protein. Proc. Natl. Acad. Sci. U.S.A. 92 (10), 4467-4471 (1995). 2. Rossi,M., Abbondanza,C., D'Arcangelo,A., Gazzerro,P., Medici,N., Moncharmont,B. and Puca,G.A. The Zn-finger domain of RIZ protein promotes MCF-7 cell proliferation. Cancer Lett. 215 (2), 229-237 (2004). 3. Grundberg,E., Carling,T., Brandstrom,H., Huang,S., Ribom,E.L., Ljunggren,O., Mallmin,H. and Kindmark,A. A deletion polymorphism in the RIZ gene, a female sex steroid hormone receptor coactivator, exhibits decreased response to estrogen in vitro and associates with low bone mineral density in young Swedish women. J. Clin. Endocrinol. Metab. 89 (12), 6173-6178 (2004). 4. C. Abbondanza et.al PNAS vol. 97 (7); 3130-3135 (March 28, 2000) 5. RB Chadwick et.al PNAS 97(6): 2662

Additional Info

Recognizes human PRDM2. Species Crossreactivity: chimpanzee. Species sequence homology: canine.