PHD4 (PH4, PH-4, Prolyl Hydroxlase Domain-containing 4, Prolyl Hydroxylase 4, HIF Prolyl Hydroxylase 4, HIFPH4, Hypoxia-inducible Factor Prolyl 4-hydroxylase, HIF Prolyl 4-hydroxylase, FLJ20262, Hypoxia Inducible Factor Prolyl Hydroxylase, P4H with Transm

Prolyl hydroxylase 4 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the prolyl hydroxylase sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. Prolyl hydroxylase is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, prolyl hydroxylases convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.\nProlyl hydroxylase 4, specifically, catalyzes the post-translational formation of 4-hydroxyproline in HIF alpha proteins. It may function as a cellular oxygen sensor and, under normoxic conditions, may targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.\n\nPositive Control: HeLa and human glioblastoma cell lysates\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P3375-30A

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Tris-citrate/phosphate [pH 7-8]

P Type

Pab

Purity

Purified by peptide immunoaffinity chromatography.

References

1. Bruick, RK and McKnight, SL. A Conserved Family of Prolyl-4-Hydroxylases That Modify HIF. Science. 294: 1337-1340 (2001)\n2. Ivan, M, et al. Biochemical Purification and Pharmacological Inhibition of a Mammalian Prolyl Hydroxylase Acting on Hypoxia-Inducible Factor. PNAS. 99(21): 13459-13464 (2002)\n3. Maxwell, PH and Ratcliffe, PJ. Oxygen Sensors and Angiogenesis. Cell & Dev. Bio. 13: 29-37 (2002)

Additional Info

This antibody is specific for prolyl hydroxylase 4. Species Crossreactivity: Human. Does not appear to work in mouse. Other species have not been tested.