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Isopentenyl-diphosphate Delta-isomerase 1, Recombinant, Human, aa1-228, His-tag (IDI1, Isopentenyl Pyrophosphate Isomerase 1, IPP Isomerase 1, IPPI1)

Cat no: I8959-76

Isopentenyl-diphosphate Delta-isomerase 1, Recombinant, Human, aa1-228, His-tag (IDI1, Isopentenyl Pyrophosphate Isomerase 1, IPP Isomerase 1, IPPI1)

IDI1, is a member of the IPP isomerase type I family and is involved in cholesterol biosynthesis. IDI1 is a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP), which is the substrates for the successive reaction that results in the synthesis of farnesyl diphosphate and, ultimately, cholesterol.\n\nSource:\nRecombinant corresponding to aa1-228 from human IDI1, fused to His-tag at N-terminal expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MMPEINTNHL DKQQVQLLAE MCILIDENDN KIGAETKKNC HLNENIEKGL LHRAFSVFLF NTENKLLLQQ RSDAKITFPG CFTNTCCSHP LSNPAELEES DALGVRRAAQ RRLKAELGIP LEEVPPEEIN YLTRIHYKAQ SDGIWGEHEI DYILLVRKNV TLNPDPNEIK SYCYVSKEEL KELLKKAASG EIKITPWFKI IAATFLFKWW DNLNHLNQFV DHEKIYRM\n\nMolecular Weight:\n~28.6kD (MALDI-TOF)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8959-76

Size

50ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 1mM DTT, 10% glycerol, 0.1M sodium chloride.

Purity

~90% (SDS-PAGE)

References

1. Hahn F M., et al. (1996) Arch Biochem Biophys. 332:30-34. 2. Zheng W., et al. (2007) 3. J Mol Biol. 366:1447-1458.

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SUPPLIER INFO

Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

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Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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