Heparan Sulfate delta (3G10 epitope)

Heparan sulfate, otherwise known as heparitin sulfate or heparin monosulfate, is a generic term describing polysaccharides which are linear and consist of N-acetylated [->4)alpha-D-GlcNpAc-(1->4) b-D-GlcAp(1->] and N-sulfated disaccharides [->4)alpha- D-GlcNpS- (1->4)-b-D-GlcAp or alpha-L-IdoAp(1->] that are arranged mainly in a segregated manner. The sulfate-rich fractions in heparan sulfate are heparin-like, though they rarely possess the sulfate density found in heparin. Approximately 25% of the total polymer is initially formed by alternating arrangements of the two disaccharide units, ->4)alpha- D-GlcNpS(1->4)UAp(1->4) alpha-D-GlcNpAc(1->4)UAp (1->4)- alpha-D-GlcAp(1->. The polymer is formed as a repeating ->4) -alpha-D-GlcNpAc (1->4)-b-D-GlcAp(1->disaccharide sequence that is attached to a serine residue of a core protein through a tetra- saccharide, glucuronosyl->galactosyl-> galactosyl->xylosyl, linkage region. It then undergoes partial N-deacetylation followed by N-sulfation of the newly exposed amino groups, partial C-5 epi- merization of D-GlcAp to L-IdoAp and O-sulfation. O-sulfates are always found in proximity to N-sulfates which enhances the clustering of the sulfate residues and the heterogeneity in chemical composition and charge density of heparan sulfate.\n\nApplications: \nSuitable for use in Flow Cytometry, Immunohistochemistry, ELISA and Western Blot. Other applications have not been tested.\n\nRecommended Dilutions:\nFlow Cytometry: 1:100-1:200 (0.5-1ug labels ~10e6 cells).\nImmunohistochemistry: 1:100-1:200\nELISA: 1:200-1:500\nOptimal dilutions to be determined by the researcher.\n\nHybridoma:\nMouse myeloma SP2/0-Ag14 with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1890-75

Size

50ug

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide. No stabilizing proteins added.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2b,k

References

1. David, G., et al., J. Cell. Biol. 119: 961-975 (1992). 2. Bai, X.M., et al., J. Histochem. Cytochem. 42: 1043-1054 (1994). 3. Nackaerts, K., et al., Int. J. Cancer (Pred. Oncol.) 74: 335-345 (1997). 4. Fuxe, K., et al., Brain Research 636: 131-138 (1994).

Additional Info

Recognizes human heparan sulfate neo-epitope, generated by digesting heparan sulfate with heparitinase from Flavobacterium heparinum (EC4.2.2.8). The desaturated hexuronate (glucuronate) that is present at the non-reducing end of the heparan sulfate fragments created by the enzyme is critical for the reactivity of the antibody. Does not react with oligosaccharides generated from chondroitin sulfates with bacterial chondroitin ABC or AC lyases or generated from heparan sulfate with heparinase (EC4.2.2.7).