EphA1, Fc Chimera, Recombinant, Human (Ephrin-A1, EPH-related Receptor Tyrosine Kinase Ligand 1, LERK-1, Immediate Early Response Protein B61, Tumor Necrosis Factor alpha-induced Protein 4, TNF alpha-induced Protein 4, EFNA1, EPLG1, LERK1, TNFAIP4)

Ephrin-A1, also known as B61 and LERK-1, is a member of the Ephrin-A family of GPI-anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin-A ligands are structurally related to the extracellular domains of the transmembrane Ephrin-B ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Human Ephrin-A1 is synthesized with an 18aa signal peptide, a 164aa mature chain, and a 23aa C-terminal propeptide which is removed prior to GPI linkage of Ephrin-A1 to the membrane. It can also be released as a soluble molecule. The mature 21-25kD human Ephrin-A1 shares 85% aa sequence identity with mouse and rat Ephrin-A1. Alternate splicing generates an additional isoform that lacks 22aa in the juxtamembrane region. This short isoform is also expressed on the cell surface and exhibits weakened binding to EphA2. Ephrin-A1 is widely expressed on endothelial and epithelial cells, particularly in the lung, intestine, liver, and skin. It is expressed on resting CD4+ T cells but is down-regulated following activation. Ligation of Ephrin-A1 on CD4+ T cells inhibits cell proliferation and activation, although soluble Ephrin-A1 can promote T cell chemotaxis. In cancer, Ephrin-A1 is expressed by tumor cells as well as on the tumor-associated vasculature. It inhibits tumor cell proliferation and migration but also supports tumor growth by promoting angiogenesis. Soluble Ephrin-A1 additionally promotes neuronal survival and neurite extension.\n\nSource:\nRecombinant corresponding to aa1-182 from human Ephrin-A1 Fc Chimera expressed in mouse myeloma cell line, NS0.\n\nMolecular Weight:\n~46kD\n\nBiological Activity:\nMeasured by its binding ability in a functional ELISA. When Recombinant Human (rh) EphA2 is coated at 2ug/ml (100ul/well), the concentration of rhEphrin-A1 Fc Chimera that produces 50% of the optimal binding response is found to be ~0.6-3ng/ml.\n\nEndotoxin: ~1EU/1ug (LAL)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3355-01D

Size

50ug

Form

Supplied as a lyophilized powder in PBS. BSA free. Reconstitute with PBS to 0.1mg/ml.

Purity

~95% (SDS-PAGE)

References

1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762. 2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165. 3.Holzman, L.B. et al. (1990) Mol. Cell. Biol. 10:5830. 4. Shao, H. et al. (1995) J. Biol. Chem. 270:5636. 5. Easty, D.J. et al. (1995) Cancer Res. 55:2528. 6. Cui, X.