D-Mannitol is one of the most abundant sugar alcohols in nature. It can be produced in the biosphere by a range of organisms such as bacteria, yeast, fungi and almost all plants. Mannitol functions as a carbohydrate storage depot, as a scavenger for reactive oxygen species and as an osmoprotectant. Recent studies have shown that increased mannitol content in plants protects them against fungal infections and improves resistance to drought and high salinity. The mannitol pathway reversibly converts fructose to mannitol and serves as the main carbohydrate cycle for fungi survival. Measurement of mannitol level is important for evaluating mannitol metabolic pathways and commercially, in developing fungi and drought resistant plants. In D-Mannitol Assay Kit, D-mannitol is converted to D-fructose by mannitol dehydrogenase in the presence of NAD to form NADH, which reduces a colorless probe to a chromogen with strong absorbance at 450nm. L-Arabitol, another sugar alcohol, also acts as substrate for mannitol dehydrogenase & undergoes similar reaction; therefore, this kit can also be used to measure L-arabitol. The D-mannitol assay kit is fast (~15 minutes), sensitive & easy to use. This assay kit can detect mannitol level less than 1 nmol/reaction (< 10uM) & can be used for a variety of sample types.
Sample Type:
Plant tissues, fungi, bacteria, food, jiuces and other beverages.
Intended Use:
The kit can detect Mannitol concentration less than 1nmol (<10uM).
Sensitivity:
D-mannitol less than 1 nmol (<10uM).
Kit Components:
Mannitol assay buffer, 100x27ml
Mannitol enzyme mix (lyophilized), 100 vials.
Mannitol substrate mix (lyophilized), 100 vials
Mannitol standard (lyophilized), 100 vials
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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