Cholesterol Esterase is found primarily in pancreas and pancreatic secretions, but in other tissues as well. Bile salts, such as cholate and its conjugates, act as stabilizers of the native polymeric form of the enzyme and can protect it from proteolytic degradation in the intestine. Cholesterol esterase is an enzyme that catalyses the hydrolysis of cholesterol and some other sterol esters to liberate cholesterol plus a fatty acid anion.
The enzyme is bound to cellular membranes of excitable tissue (synaptic junction, endoplasmic reticulum, etc.). It is believed to be associated with nerve impulse conduction.
Source:
Candida rugosa
Activity:
6 U/mg
One unit catalyses the hydrolysis of one micromole of cholestrol ester per minute in the presence of Sodium cholate at 37C, pH 6.7
Applications:
Suitable for use in ELISA and Functional Assays. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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