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V5 Epitope Tag GKPIPNPLLGLDST (Pk-tag, Paramyxovirus, SV5)

Cat no: V0500-00B

Supplier: United States Biological
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The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence. Hybridoma: Mouse Anti-V5-tag is a mouse monoclonal antibody, affinity purified from culture supernatant of hybridoma cells grown in a bioreactor. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:5000-1:10,000 Western Blot: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: V0500-00B
Hosts: Mouse
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in 10mM PBS, pH 7.2.
P type: Mab
Isotype: IgG1
Purity: Purified by immunoaffinity chromatography.
Additional info: Detects (GKPIPNPLLGLDST) tag fusion protein.

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