Tyrosine hydroxylase play an important role in the synthesis of dopamine and other catecholamines. The enzyme's activity is regulated in the level of transcription and translation, as well as posttranscriptional modifications. Phosphorylation at Ser40 may play a key role in regulation of the catalytic activity of the enzyme.
Applications:
Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended Dilution:
Immunofluorescence: 1:50-1:100
Western Blot: 1:200-1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.