Troponin (Tn) is the central regulatory protein of striated muscle contraction. Tn consists of three components: TnI which is the inhibitor of actomyosin ATPase, TnT which contains the binding site for tropomyosin and TnC. The binding of calcium to TnC abolishes the inhibitory action of Tn on actin filaments, and as a result of calcium ion uptake by troponin C, the elements of the muscle thin filament undergo a series of allosteric changes which allow the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension.
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.