Neurotrophins activate tyrosine kinase receptors of the Trk family (TrkA, TrkB and TrkC) and they all share a common low affinity receptor (p75NTR or p75). Although p75NTR does not appear to directly transduce NGF signals, this receptor appears to increase the responsiveness of the trk receptors and plays a role in the retrograde transport of neurotrophin signals from axon terminals to the cell body. The expression of trk gene neurotrophin receptors in mouse is tabulated by Barbacid (1994). NGF binds with high affinity and activates the TrkA tyrosine kinase receptor, which is apparently responsible for signal transduction. BDNF activates the TrkB tyrosine kinase receptor, which it shares with NT-4. NT-3 activates the TrkC tyrosine kinase receptor, but can also activate TrkA and TrkB receptors in certain cell systems. NT-4 primarily activates the TrkB tyrosine kinase receptor, which it shares with BDNF.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:100-1000
Immunohistochemistry (Frozen): 1:50-200,
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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