Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organism. It is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family, which is expressed in a variety of human tissues. Similar to TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells. Its receptors include the death domain containing proteins DR4 and DR5, both of which mediate TRAIL induced cell death. Two decoy receptors, DcR1 and DcR2, have also been identified. Both of these proteins contain an extracellular TRAIL binding domain but lack intracellular signaling domains. Over-expression of either DcR1 or DcR2 attenuates TRAIL-induced apoptosis.
Rabbit polyclonal antibodies were raised against peptide sequences corresponding to each of the target proteins.
Kit Components:
TRAIL (Rb x Hu): 1x25ug
DR4 (Rb x Hu): 1x25ug
DR5 (Rb x Hu): 1x25ug
DcR1 (Rb x Hu): 1x25ug
DcR2 (Rb x Hu): 1x25ug
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control:
HeLa whole cell lysate
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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