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TRA-1-60 (TRA-1, Tumor Rejection Antigen 1)

Cat no: T8115-05

Supplier: United States Biological
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TRA-1-60 specific antibody reacts with podocalyxin, a sialomucin which is a member of the CD34 family. It is expressed on human teratocarcinoma cells (EC), embryonic stem (ES) and germ cells. It is not expressed on differentiated cells. TRA-1-60R is a neauraminidase-resistant epitope variant of TRA-1-60. TRA-1-60 expression is proportional to tumor growth. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug labels 10e6 cells Immunohistochemistry: Formalin fixed paraffin embedded Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: T8115-05
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 25ug
Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
P type: Mab
Isotype: IgM,k
Purity: Purified by affinity chromatography.
References: 1. Schopperle, W.M., and DeWolf, W.C. (2007). Stem Cells 25, 723-730. 2. Badcock, G., et al. (1999). Cancer Res. 59, 4715-4719. 3. Andrews, P.W., et al. (1984). Hybridoma 3, 347-361.
Additional info: Recognizes human TRA-1-60.

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