Home  >  Products  >  TRA-1-60 (TRA-1, Tumor Rejection Antigen 1) (PE)

TRA-1-60 (TRA-1, Tumor Rejection Antigen 1) (PE)

Cat no: T8115A

Supplier: United States Biological
0 reviews | Write a Review Pencil
TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells. The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors. Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan. Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope. The original TRA-1-60 antibody is now known to include two distinct specificities that recognize a neuraminidase resistant epitope [TRA-1-60(R)] or a neuraminidase-sensitive epitope [TRA-1-60(S)]. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Designed to quantitatively determine the percentage of cells bearing TRA-1-60(R) within a population and qualitatively determine the density of TRA-1-60(R) on cell surfaces by flow cytometry. Cell surface expression of TRA-1-60(R) is determined by flow cytometry using 488 nm wavelength excitation and monitoring emitted fluorescence with a detector BG01V cells were stained with PE-conjugated anti-human TRA-1-6 (filled histogram) or isotype control (open histogram). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: T8115A
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100Tests
Form: Supplied as a lyophilized powder in 25ug antibody, 1ml saline, 0.5% BSA, 0.1% sodium azide.
P type: Mab
Isotype: IgM
Purity: Human embryonal carcinoma cell line, 2102E9
References: 1. Andrews, P. et al. (1984) Hybridoma 3:347. 2. Thomson, J. et al. (1998) Science 282:1145. 3. Giwercman, A. et al. (1993) Cancer 72:1308. 4. Marrink, J. et al. (1991) Int. J. Cancer 49:368. 5. Badcock, G. et al. (1999) Cancer Res. 59:4715. 6. Schopperle, W. & W. DeWolf (2007) Stem Cells 25:723.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*

Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave