TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells. The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors. Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan. Subsequent
evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope. The original TRA-1-60 antibody is now known to include two distinct specificities that recognize a neuraminidase resistant epitope [TRA-1-60(R)] or a neuraminidase-sensitive epitope [TRA-1-60(S)].
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: Designed to quantitatively determine the percentage of cells bearing TRA-1-60(R) within a population and qualitatively determine the density of TRA-1-60(R) on cell surfaces by flow cytometry. Cell surface expression of TRA-1-60(R) is determined by flow cytometry using 488 nm wavelength excitation and monitoring emitted fluorescence with a detector BG01V cells were stained with PE-conjugated anti-human TRA-1-6 (filled histogram) or isotype control (open histogram).
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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