Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defence to many pathogens. In Drosophila Toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLR identified as type I transmembrane signalling receptors with pattern recognition capabilities, have
been implicated in the innate host defence to pathogens. Mammalian responsiveness to some bacterial products may be mediated by combinations of TLRs, for example a co-operative effect is observed between TLR1 (CD281) and TLR2 (CD282). TLR1 is expressed by monocytes and dendritic cells.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: 1:50
Optimal dilutions to be determined by the researcher.
Storage and Stability:
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.