Tetromycin A is an unusual tetronic acid structurally related to kijanimicin, chlorothricin, saccharocarcin, tetrocarcin and versipelostatin. Tetromycin A has pronounced activity against antibiotic susceptible and resistant Gram positive bacteria including MRSA. Limited availability has restricted further investigation of this metabolite in the literature. Several members of this class have received considerable literature focus. Versipelostatin was shown to inhibit transcription from the promoter of GRP78, a gene that is activated as part of a stress signalling pathway under glucose deprivation resulting in unfolded protein response (UPR). The UPR-inhibitory action was seen only in conditions of glucose deprivation and caused selective and massive killing of the glucose-deprived cells. Tetrocarcin A appears to target the phosphatidylinositide-3'- kinase/Akt signalling pathway.
Soluble in ethanol, methanol, DMF or DMSO. Moderately soluble in water.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with ethanol, methanol, DMF or DMSO. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.