Testosterone is a steroid hormone secreted by interstitial or Leydig cells of the normal testis. The determination of testosterone concentrations in the body fluids is of great value for endocrinological investigations. Useful in identification of testicular tumors arising from interstitial cells.
Applications:
Suitable for use ELISA. Other applications not tested.
Recommended Dilution:
Titer: 1:2000 (by EIA)
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
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