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TCR, Cbeta1 (T Cell Receptor) (FITC)

Cat no: T2040-04A

Supplier: United States Biological
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Human T cell receptor (TCR) is a T cell surface expressed heterodimer of a/b or g/d chains. TCR's recognize antigen peptides bound to MHC molecules forming the basis of antigen specific response by T cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50; 80ul labels 5x10e5 human Jurkat cells. Optimal dilutions to be determined by the researcher. FACS Protocol: 1. Five x 10e5 human Jurkat cells were washed and preincubated 45 minutes with 80ul T2040-04A at 1:50 (10ug/ml). 2. Cells were then washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 0.72 log 10 fluorescent units when compared to a Mouse IgG2a/FITC negative control at a similar concentration. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: T2040-04A
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein A affinity chromatography.
References: 1. J.L. Viney, et al, (1992) Hybridoma 11: 701-713. 2. A. Weiss, (1993) Cell 73: 209-212. 3. M.M. Daris, (1995) Nature 375: 104. 4. S. Valitutti, et al, (1995) Nature 375: 148-151.
Additional info: Recognizes human C beta 1 TCR gene product. Reacts with 50-75% of T cells in normal human blood. Is a mitogenic for T cells expressing TCR C beta 1.

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